CYTOTOXIC EFFECTS OF THEOBROMINE DERIVED COPPER NANOPARTICLES ORAL CANCER CELLS VIA P53 ACTIVATION

Abstract

Background: Oral cancer remains a major global concern , with limited success from conventional therapies due to chemoresistance and systemic toxicity.Phytochemical assisted nanoparticles due to chemoresistance and systemic toxicity.Phytochemical assisted nanoparticles reflect a biocompatible alternative with multi potency.This study investigates the cytotoxicity and molecular effect of Theobromine derived copper nanoparticles (Theobro-CuONPs) in KB oral cancer cells

Objective: To investigate the cytotoxicity of Theobro-CuONPs by emphasizing p53 mediated apoptosis and inflammatory signalling in a gene expression profile in KB cells

Methods: Theobro-CuONPs have been synthesized employing theobromine , a natural and green reducing agent. The synthesized nanoparticles were characterized by standard techniques. The cytotoxic effect was determined by performing an MTT assay , whereas the expression of p53 , Bax , Bcl-2 , Caspase-3 , TNF - α , IL-6 and IL-10 genes was assessed by quantitative real time PCR in treated vs control KB cells.

Results: Theobro-CuONPs decreased cell viability more than theobromine or CuO alone in a dose-dependent manner. Gene expression profiling indicated that while the expressions of p53 , Bax and Caspase-3 were upregulated , those of Bcl-2 , TNF-α , IL-6 and IL-10 were downregulated . Thus , the intrinsic apoptotic pathway got activated while suppressing inflammatory responses.

Conclusion: Theobro- CuONPs exhibit major anticancer effects by altering the expression of key apoptotic and inflammatory genes , thus proving their immense potential as a green , biocompatible candidate for oral cancer therapy