CYTOTOXIC EFFECT AND FREE RADICAL SCAVENGING ACTIVITY OF ANDROGRAPHIS PANICULATA STEM MEDIATED SELENIUM NANOPARTICLES

Abstract

Introduction:Nanotechnology is revolutionizing biomedical sciences, particularly in synthesizing selenium nanoparticles (SeNPs) using plant-based methods. These plants contain metabolites like proteins and flavonoids that act as reducing agents to generate and stabilize nanoparticles. SeNPs, known for their immunomodulatory and antioxidant properties, are significant in medicine, agriculture, and environmental applications. Andrographis paniculata, a traditional medicinal plant, shows promising cytotoxic and free radical scavenging activities when used in SeNP synthesis. Materials and methods : The study investigates the cytotoxic effect and free radical scavenging activity of selenium nanoparticles synthesized using Andrographis paniculata. The plant extract, prepared by filtering the leaves, was mixed with sodium selenite to produce the nanoparticles. These nanoparticles were then tested on brine shrimp nauplii to assess their toxicity at various concentrations. Observations and data calculation were performed to evaluate the mortality rate, providing insight into the potential biomedical applications of the synthesized nanoparticle Results: The study evaluates selenium nanoparticles (SeNPs) synthesized using Andrographis paniculata for their cytotoxic and antioxidant properties. The SeNPs exhibit dose-dependent antioxidant activity, with increasing efficacy at higher concentrations, comparable to standard antioxidants. Although the standard shows greater antioxidant activity at lower doses, A. paniculata SeNPs demonstrate significant inhibition of oxidative agents, suggesting their potential for biomedical applications. Additionally, the SeNPs show minimal cytotoxicity and promote wound healing, while the nauplii assay reveals a time and dose-dependent cytotoxic effect, highlighting their therapeutic versatility. Conclusion: The study demonstrates that Andrographis paniculata stem extract effectively synthesizes selenium nanoparticles (SeNPs) with strong cytotoxic and antioxidant properties. These SeNPs show significant potential as therapeutic agents in cancer treatment due to their notable cytotoxic effects against various cancer cell lines. Additionally, their robust free radical scavenging activity underscores their potential as antioxidants. The dual functionality of A. paniculata stem-mediated SeNPs offers a promising avenue for developing novel anticancer and antioxidant therapies. Further research is essential to understand their mechanisms and assess in vivo efficacy and safety.